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Fig. 9 | Arthritis Research & Therapy

Fig. 9

From: Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Fig. 9

Effects of CKD-L in the suppression assay using induced regulatory T (Treg) cells and effector T (Teff) cells from RA patients (n = 3). Treg cells induced from RA patients were mixed with carboxyfluorescein succinimidyl ester (CFSE)-labeled Teff cells at ratios of 1:1, 0.3:1, and 0:1. The mixed cells were incubated with vehicle or HDAC inhibitors (0.01 to 5 μM) in the presence of Dynabeads® human T-activator CD3/C28 (one bead to four cells) for 3 days. a The proliferation of Teff cells was assessed by CFSE dilution using flow cytometry (representative data). b Suppression ratio (fold inhibition of cell proliferation by HDACi vs vehicle) at the ratio of Treg:Teff = 1:1. *p <0.001, **p < 0.05, vs vehicle

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