Fig. 1
From: Beneficial effect of resveratrol on phenotypic features and activity of osteoarthritic osteoblasts
Effect of resveratol (RSV) on sirtuin 1 (Sirt1) and P300 levels. a Representative immunohistochemical determination of Sirt1 protein expression in normal subchondral bone tissues and in osteoarthritis (OA). b Sirt1 expression by normal (n = 3) and OA (n = 6) ex vivo subchondral bone explants. c Sirt1 expression by normal (n = 9) and OA osteoblasts (Ob) (n = 21). d Confluent OA Ob were treated with increasing doses of RSV for 24 h and lysed in TRIzolTM for qRT-PCR analysis of Sirt1 messenger RNA expression (n = 5). e Quantification of Sirt1 levels as detected in Fig. 2c. f Sirt1 activity analysis in OA Ob cell lysates in response to RSV (n = 3). GAPDH glyceraldehyde-3-phosphate dehydrogenase, CTRL control