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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Beneficial effect of resveratrol on phenotypic features and activity of osteoarthritic osteoblasts

Fig. 2

Effect of resveratol (RSV) EP-300 and leptin expression, extracellular signal-regulated kinase (Erk)1/2 and Smad1/5/8 signaling activities in osteoarthritis (OA) osteoblasts (Ob). Confluent OA Ob were treated with increasing doses of RSV for 24 h and lysed in TRIzolTM for qRT-PCR analysis. a EP-300 messenger RNA (mRNA) expression (n = 6). b EP-300 protein abundance in response to 24 h of RSV treatment at 500 nM. c Western blot analysis of EP-300 and sirtuin 1 (Sirt1) in response to 24 h of RSV treatment at 500 nM. d Leptin mRNA expression (n = 6). Results are given as the mean value ± SEM of markers relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). e Representative western blot analysis of OA Ob treated or not with 500 nM of RSV for 1 h. Phosphorylated and non-phosphorylated Erk1/2, phosphorylated Smad1/5/8, and β-actin were detected by western blot analysis (n = 6 experiments). f Quantification of phosphorylated and non-phosphorylated Erk1/2 levels as detected in e. g Specific inhibition of RSV-dependent phosphorylated Erk1/2 by PD98059. CTRL control, MAPK mitogen-activated protein kinase

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