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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Beneficial effect of resveratrol on phenotypic features and activity of osteoarthritic osteoblasts

Fig. 3

Effect of resveratol (RSV) on proliferation, bax/bcl2, runx2/cbfa1, and peroxisome proliferator-activated receptor γ (PPARγ) expression in osteoblasts (Ob) in osteoarthritis (OA). a OA Ob were plated at 10,000 cells/cm2 and allowed to attach overnight in BGJ medium containing 10% FBS. Cells were then treated with the same medium with 0.5% FBS for 24 h prior to receiving increasing doses of RSV (10 , 50 , 100, 500 or 1000 nM) or the vehicle in the same medium for another incubation of 24 h. Cell proliferation was assessed by the MTT assay (n = 5). Confluent OA Ob were treated with increasing doses of RSV for 24 h and lysed in TRIzolTM for qRT-PCR analysis. b BAX/BCL2 messenger RNA (mRNA) expression (n = 6). c Runx2/Cbfa1 mRNA expression (n = 7). d PPARγ mRNA expression (n = 6). Results are given as the mean value ± SEM of markers relative to glyceraldehyde-3-phosphate dehydrogenase

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