Fig. 5

Effect of resveratol (RSV) on Wnt/β-catenin signaling activity and mineralization in osteoblasts (Ob) in osteoarthritis (OA). OA osteoblasts were stimulated with increasing doses of RSV (10, 50, 100, 500 or 1000 nM) for up to 28 days. a Representative alizarin red staining of normal Ob. b Quantification of alizarin red staining as a function of time and chronic effect of RSV exposure in OA Ob (n = 6). Cells were treated for 4 h with vehicle or increasing doses of RSV. c TOPflash activity in OA Ob in response to parental and Wnt3a conditioned medium either in the presence or absence of RSV for 24 h. Values are reported relative to values in parental samples (n = 5 experiments). d Western blot analysis of β-catenin expression in OA Ob in response to Wnt3a in the presence of RSV. Representative of four experiments. e Western blot analysis of the effect of PD98059 on RSV-dependent β-catenin activation in OA Ob. Representative of three experiments. CTRL control