Fig. 3
From: Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Suppression of inflammatory cytokine gene expression in splenic T cells by Dow2. Quantification of mRNA levels for IL-1α, IL-2, IFN-γ, T-bet, IL-17, IL-10, and Foxp3 in mouse splenocytes incubated with Dow2, 17A2, or rat IgG isotype control. Total RNA was extracted from cells, reverse transcribed, and amplified by qRT-PCR with primers for these genes and GAPDH. Results present the relative expression of each gene compared to cells incubated with rat IgG (ΔΔCt; splenocytes + rat IgG = 1). Representative data from four independent experiments with similar results are shown. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.005, ***P < 0.0005. 1 splenocytes + rat IgG isotype control, 2 splenocytes + Dow2, 3 splenocytes + 17A2, n.s. not significant