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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Hydroxychloroquine inhibits CD154 expression in CD4+ T lymphocytes of systemic lupus erythematosus through NFAT, but not STAT5, signaling

Fig. 6

HCQ treatment did not inhibit CD154 expressed through the IL-15-mediated STAT5 signaling pathway. a CD4+ T cells from SLE patients were cultured in different concentrations of HCQ (1 × = 600 ng/ml) for 24 hours and then activated with PHA (1.5 μg/ml), IL-15 (75 ng/ml), or a combination for 6 or 20 hours. Flow cytometry was used to measure CD154 expression. Histogram plots shown are representative of five similar experiments (upper panel). Flow cytometric results are shown as a percentage of CD154 expression of CD4+ T cells. Data are mean ± SEM of duplicate samples and are representative of five independent experiments (lower panel). b Jurkat cells were pretreated with the indicated concentration of HCQ (1 × = 600 ng/ml) for 24 hours and then stimulated with IL-15 (75 ng/ml) for 20 min or left unstimulated (W/O). Western blot analysis was performed using cell extracts and antibodies specific for phosphorylated STAT5. Results shown are representative of six similar experiments (upper panel). Western blot results are shown as a normalized phosphorylated STAT5/actin ratio. Data are mean ± SEM of duplicate samples and are representative of six independent experiments (lower panel). HCQ hydroxychloroquine, IL interleukin, ns not significant, PHA polyhydroxyalkanoates, STAT5 signal transducer and activator of transcription 5

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