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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

Fig. 4

MicroRNA (miR, miRNA)-488 and miR-920 inhibit monosodium urate (MSU)-induced interleukin (IL)-1β protein expression in monocytic THP-1 cells. The miRNA mimics or negative control (NC) mimics (50 nM) were transfected into THP-1 cells using Lipofectamine RNAiMAX reagent in accordance with the manufacturer’s instructions. After 24 h of transfection, cells were stimulated for 3 h with 0.5 μM 12-myristate 13-acetate. Then, cells were washed and stimulated with 250 μg/ml MSU crystals for 24 h to detect the production of IL-1β. After the treatment, the cells were collected and analyzed by quantitative real-time polymerase chain reaction (a) or Western blotting (c). The cell culture supernatants were collected to detect the concentrations of IL-1β by enzyme-linked immunosorbent assay (b). d Densitometric analysis of immunoblot band intensities for IL-1β normalized by β-actin. Values are expressed as mean ± SEM of three independent experiments. # P < 0.05 versus NC, *P < 0.05 versus MSU stimulation alone

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