Fig. 5

MicroRNA (miR, miRNA)-488 and miR-920 directly target the 3′-untranslated region (3′-UTR) of interleukin (IL)-1β. Sequence alignments of the miRNA base pair site in the 3′-UTR of IL-1β messenger RNA (mRNA) are shown in the left figures (a-e). The “seed” sequence of miRNA that is complementary to IL-1β is shown in capital letters in the dashed boxes. The mutant sequence (mut) is identical to the wild-type sequence (wt), except the mutated nucleotides are shown in red. Results of luciferase assays with HEK 293 T cells are shown in the right figures (a-e). Cells were cotransfected with wt/mut 3′-UTR with miRNA mimics or negative control as indicated. Twenty-four hours after transfection, luciferase activity was detected using a dual-luciferase reporter assay system according to the manufacturer’s instructions. Values are expressed as mean ± SEM of three independent experiments, each of which was run in triplicate. *P < 0.05 versus scrambled group. RLU Relative light units