Skip to main content


Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: S100A8/A9 increases the mobilization of pro-inflammatory Ly6Chigh monocytes to the synovium during experimental osteoarthritis

Fig. 2

Intra articular injection of S100A8 leads to local induction of monocytes in the synovium. a-f mRNA expression of monocyte marker Ly6C (a), macrophage marker F4/80 (B), Ly6Chigh chemokine CCL2 (c), and Ly6Chigh chemokine receptor CCR2 (d) are increased in the synovium of mice 24 h after intra articular injection with S100A8, compared to BSA-injected controls, whereas Ly6Clow chemokine CX3CL1 (e), and Ly6Clow chemokine receptor CX3CR1 (f) are not affected after S100A8 administration, as analyzed using RT-qPCR. Data represent mean ± SD of six individual mice. *Significantly different from treated control (*p < 0.05). g, h HE staining on histological sections of the joint 24 h after intra-articular (i.a.) injection with S100A8 demonstrated strong presence of inflammatory cells with monocyte-like morphology in the synovial lining layer of the knee joints (g), whereas BSA injection as control resulted in less pronounced presence of inflammatory cells (h). Ct cycle threshold

Back to article page