Fig. 3

The number of Ly6C^high monocytes decreases during collagenase-induced osteoarthritis (CiOA) in the bone marrow (BM) of wild-type (WT) but not S100a9^-/- mice, but not in the blood and spleen of WT or S100a9^-/- mice. a The relative number of myeloid cells in the complete contralateral femur of WT mice was decreased early during CiOA compared to saline-injected controls, whereas no effects were found in S100a9^-/- mice at CiOA day 7. b The relative number of Ly6C^high monocytes in the complete contralateral BM of WT mice was also decreased early during CiOA compared to saline-injected control animals, whereas no effect was observed in S100A9^-/- mice. c Gating strategy of monocyte subsets in the BM, using flow cytometry. d, e The relative number of Ly6C^high and Ly6C^low monocyte subpopulations was not affected in the peripheral blood (d), and spleen (e) at CiOA day 7 in WT or S100a9^-/- mice when compared to saline-injected control animals. f, g Gating strategy of monocyte subsets in the peripheral blood (f) and spleen (g), measured using flow cytometry. Data represent mean ± SD of 5–7 individual mice per group (except for WT mice on day 7 where n = 11–12 animals). *Significantly different from saline-injected control (*p < 0.05, **p < 0.01, ***p < 0.001)