S100A8/A9 increases the mobilization of pro-inflammatory Ly6C high monocytes to the synovium during experimental osteoarthritis

Table 1 Overview of animals used in experiments

Experiment	Readout	Time point	Number of animals per group and strains
CiOA/control	Monocyte populations and RNA expression synovium and BM	Day 7	6 CiOA/6 control C57BL/6
CiOA/control	Monocyte populations and RNA expression synovium and BM	Day 7	6 CiOA/6 control S100a9^-/-
CiOA/control	BM count	Day 7, 21, and 42	6 CiOA/7 control C57BL/6 (day 7)
			6 CiOA/7 control C57BL/6 (day 21)
			7 CiOA/7 control C57BL/6 (day 42)
CiOA/control	Monocyte populations and BM count	Day 7	5 CiOA/5 control C57BL/6
CiOA/control	Monocyte populations and BM count	Day 7	5 CiOA/5 control S100a9^-/-
CiOA	Immunohistologic evaluation (and serum for protein analysis)	Day 7, 21, and 42	10 per time-point C57BL/6 (day 7)
			10 per time-point S100a9^-/- (day 7)
			10 per time-point C57BL/6 (day 21)
			10 per time-point S100a9^-/- (day 21)
			10 per time-point C57BL/6 (day 42)
			10 per time-point S100a9^-/- (day 42)
i.a. injection with S100A8/BSA	Immunohistologic evaluation	Day 1	8 C57BL/6
i.a. injection with S100A8/BSA	RNA expression	Day 1	6 C57BL/6

CiOA collagenase-induced osteoarthritis, i.a., intra-articular, BM bone marrow