Experiment | Readout | Time point | Number of animals per group and strains |
---|---|---|---|
CiOA/control | Monocyte populations and RNA expression synovium and BM | Day 7 | 6 CiOA/6 control C57BL/6 |
6 CiOA/6 control S100a9-/- | |||
CiOA/control | BM count | Day 7, 21, and 42 | 6 CiOA/7 control C57BL/6 (day 7) |
6 CiOA/7 control C57BL/6 (day 21) | |||
7 CiOA/7 control C57BL/6 (day 42) | |||
CiOA/control | Monocyte populations and BM count | Day 7 | 5 CiOA/5 control C57BL/6 |
5 CiOA/5 control S100a9-/- | |||
CiOA | Immunohistologic evaluation (and serum for protein analysis) | Day 7, 21, and 42 | 10 per time-point C57BL/6 (day 7) |
10 per time-point S100a9-/- (day 7) | |||
10 per time-point C57BL/6 (day 21) | |||
10 per time-point S100a9-/- (day 21) | |||
10 per time-point C57BL/6 (day 42) | |||
10 per time-point S100a9-/- (day 42) | |||
i.a. injection with S100A8/BSA | Immunohistologic evaluation | Day 1 | 8 C57BL/6 |
i.a. injection with S100A8/BSA | RNA expression | Day 1 | 6 C57BL/6 |