Table 2 Primer sequences of mouse inflammatory and mobilization markers
| Â | Sense | Antisense |
|---|---|---|
Reference gene | ||
  Gapdh | 5′-ggcaaattcaacggcaca-3′ | 5′-gttagtggggtctcgctcctg-3′ |
Inflammation marker | ||
  S100A8 | 5′-tgtcctcagtttgtgcagaatataaat-3′ | 5′-tttatcaccatcgcaaggaactc-3′ |
  S100A9 | 5′-ggcaaaggctgtgggaagt-3′ | 5′-ccattgagtaagccattcccttta-3′ |
Mobilization markers | ||
  CCL2 | 5′-ttggctcagccagatgca-3′ | 5′-cctactcattgggatcatcttgct-3′ |
  CX3CL1 | 5′-gtgccattgtcctggagac-3′ | 5′-catttctccttcgggtcag-3′ |
  KC | 5′-tggctgggattcacctcaa-3′ | 5′-gagtgtggctatgacttcggttt-3′ |
  MIP-1α | 5′-caagtcttctcagcgccatatg-3′ | 5′-tcttccggctgtaggagaagc-3′ |
Cell markers | ||
  CX3CR1 | 5′-gagtatgacgattctgctgagg-3′ | 5′-cagaccgaacgtgaagacgag-3′ |
  CCR2 | 5′-ctatctgctcaacttggccatct-3′ | 5′-tgagcccagaatggtaatgtga-3′ |
  Ly6C | 5′-gcagtgctacgagtgctatgg-3′ | 5′-actgacgggtctttagtttcctt-3′ |
  F4/80 | 5′-aatcctgtgaagatgtgg-3′ | 5′-gagtgttgatgcaaatgaag-3′ |
Adhesion molecules | ||
  LFA1 | 5′-gaatgtatgaagggcaaagtc-3′ | 5′-gcagcaaactggtaggaag-3′ |
  VCAM | 5′-ccaagtctctccaaaagatatacagctt-3′ | 5′-atgacggtgtctccctct-3′ |
  L-selectin | 5′-actgctctgttgtgacttcc-3′ | 5′-tgtatggcgactaaatctgtg-3′ |
  PECAM1 | 5′-tccctgggaggtcgtccat-3′ | 5′-gaacaaggcagcggggttta-3′ |
  VE-cadherin | 5′-tcctcttgcatcctcactatcaca-3′ | 5′-gtaagtgaccaactgctcgtgaat-3′ |