Advertisement
Table 2 Primer sequences of mouse inflammatory and mobilization markers
| Sense | Antisense | |
|---|---|---|
| Reference gene | ||
| Gapdh | 5′-ggcaaattcaacggcaca-3′ | 5′-gttagtggggtctcgctcctg-3′ |
| Inflammation marker | ||
| S100A8 | 5′-tgtcctcagtttgtgcagaatataaat-3′ | 5′-tttatcaccatcgcaaggaactc-3′ |
| S100A9 | 5′-ggcaaaggctgtgggaagt-3′ | 5′-ccattgagtaagccattcccttta-3′ |
| Mobilization markers | ||
| CCL2 | 5′-ttggctcagccagatgca-3′ | 5′-cctactcattgggatcatcttgct-3′ |
| CX3CL1 | 5′-gtgccattgtcctggagac-3′ | 5′-catttctccttcgggtcag-3′ |
| KC | 5′-tggctgggattcacctcaa-3′ | 5′-gagtgtggctatgacttcggttt-3′ |
| MIP-1α | 5′-caagtcttctcagcgccatatg-3′ | 5′-tcttccggctgtaggagaagc-3′ |
| Cell markers | ||
| CX3CR1 | 5′-gagtatgacgattctgctgagg-3′ | 5′-cagaccgaacgtgaagacgag-3′ |
| CCR2 | 5′-ctatctgctcaacttggccatct-3′ | 5′-tgagcccagaatggtaatgtga-3′ |
| Ly6C | 5′-gcagtgctacgagtgctatgg-3′ | 5′-actgacgggtctttagtttcctt-3′ |
| F4/80 | 5′-aatcctgtgaagatgtgg-3′ | 5′-gagtgttgatgcaaatgaag-3′ |
| Adhesion molecules | ||
| LFA1 | 5′-gaatgtatgaagggcaaagtc-3′ | 5′-gcagcaaactggtaggaag-3′ |
| VCAM | 5′-ccaagtctctccaaaagatatacagctt-3′ | 5′-atgacggtgtctccctct-3′ |
| L-selectin | 5′-actgctctgttgtgacttcc-3′ | 5′-tgtatggcgactaaatctgtg-3′ |
| PECAM1 | 5′-tccctgggaggtcgtccat-3′ | 5′-gaacaaggcagcggggttta-3′ |
| VE-cadherin | 5′-tcctcttgcatcctcactatcaca-3′ | 5′-gtaagtgaccaactgctcgtgaat-3′ |
