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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: The caspase-8/RIPK3 signaling axis in antigen presenting cells controls the inflammatory arthritic response

Fig. 2

Caspase-8 deficiency alters synovial populations in the circulation and the joints at steady state. a, b Blood from naïve 10–12-week-old male Casp8 flox/flox (control, n = 4), Cre LysM Casp8 flox/flox (n = 5) and Cre CD11c Casp8 flox/flox (n = 5) mice were analyzed by flow cytometric analysis. a Fluorescence-activated cell sorting (FACS) plots from the naïve circulation. Red arrows denote the sequential gated population (red boxes). Black arrows denote he sequential non-gated population. b Cellular distribution represented as the percentage of circulating CD45+ cells. c, d Ankles from naïve 10–12-week-old male Casp8 flox/flox (control, n = 3), Cre LysM Casp8 flox/flox (n = 3) and Cre CD11c Casp8 flox/flox (n = 4) mice were analyzed by flow cytometric analysis. c FACS plots from a naïve joint. Red arrows denote the sequential gated population (red boxes). Black arrows denote the sequential non-gated population. d Synovial cell distribution presented as numbers of cells. e Proportion of macrophages (CD11b+CD64+) that are major histocompatibility complex (MHC) II+ and MHC II. Data are means ± SEM, representative of two individual studies and differences between control and Cre LysM Casp8 flox/flox or Cre CD11c Casp8 flox/flox mice were compared by the Mann-Whitney test: *p < 0.05; **p < 0.005. Casp8 caspase-8, NK natural killer, DC dendritic cells

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