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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: The caspase-8/RIPK3 signaling axis in antigen presenting cells controls the inflammatory arthritic response

Fig. 5

Receptor-interacting serine-threonine kinase 3 (RIPK3) deletion reverses the dyregulation of the joint induced by caspase-8 deficiency under steady state and arthritic conditions. a-d Ankles from naïve 10–12-week-old male Casp8 flox/flox (control, n = 5), Cre CD11c Casp8 flox/flox (n = 5) and RIPK3 –/– Cre CD11c Casp8 flox/flox (n = 3) mice were analyzed by flow cytometric analysis. a Day-0 synovial cell distribution presented as numbers of cells. b Day-0 proportion of macrophages (CD11b+CD64+) that are major histocompatibility complex (MHC) II+ and MHC II. c Day-0 CD36 expression on MHC II+ macrophages (CD11b+CD64+MHCII+), MHC II macrophages (CD11b+CD64+MHCII-) and CD11b+ dendritic cells (DCs) (CD11c+CD11b+CD64-MHCII+). d Day-0 CD206 expression on MHC II+ macrophages, MHC II macrophages and CD11b+ DCs. e-h Ankles from 10–12-week-old male control (n = 4), Cre CD11c Casp8 flox/flox (n = 4) and RIPK3 –/– Cre CD11c Casp8 flox/flox (n = 4) mice on day 3 post induction of K/BxN serum-transfer-induced arthritis were analyzed by flow cytometric analysis. e Day-3 synovial cell distribution represented as numbers of cells. f Day-3 proportion of macrophages that are MHC II+ and MHC II. g Day-3 CD36 expression on MHC II+ macrophages, MHC II macrophages and CD11b+ DCs. h Day-3 CD206 expression on MHC II+ macrophages, MHC II macrophages and CD11b+ DCs. Data are means ± SEM, representative of two individual studies and are compared between control and Cre CD11c Casp8 flox/flox mice by the Mann-Whitney test: # p < 0.05; ## p < 0.005 and between Cre CD11c Casp8 flox/flox and RIPK3 –/– Cre CD11c Casp8 flox/flox mice by the Mann-Whitney test: *p < 0.05

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