Fig. 5

Influence of SLE and healthy control MPs on MP-induced ROS production and degranulation of PMNs. Leukocytes from a healthy control were suspended in a medium containing 25% v/v of normal human serum. DHR was added to the suspensions, and the cells were stimulated with LPS in combination with MPs from 20 SLE patients and 10 healthy controls for 30 min at 37 °C before flow cytometry. (a) The resulting median fluorescence intensity (MFI) after subtraction of background fluorescence (unstimulated cells). Bars represent median values. Comparisons between groups using Mann–Whitney U test (b) Content of neutrophil gelatinase-associated lipocalin (NGAL) in the supernatants of leukocytes stimulated with MPs and LPS in combination shown as median values after subtraction of the background (unstimulated cells). HC healthy control, SLE systemic lupus erythematosus