Fig. 1

CD11c⁺ B cells were increased in cGVHD mice. B6 mice (n = 5) received an intraperitoneal injection of 5 × 10⁷ splenocytes from Bm12 or B6 mice; spleens and serum were collected at day 14 for flow analysis and the antibodies examination. a Weight and cell counts of the spleen were taken 14 days later. The figure shows spleen photographs, spleen weight, and absolute number of splenocytes. b Serum antichromatin total IgG and IgG subtype titers were examined by ELISA. c, d Flow cytometric analysis of CD11c⁺CD19⁺ cells (c) and CD11c⁺CD138⁺ cells (d) in total spleen. Values are shown as the mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001. Abs antibodies, IgG immunoglobulin G, OD optical density