Fig. 2

CD11c⁺ plasma cells in cGVHD mice produced antichromatin antibodies after stimulation in vitro. CD11c⁺CD138⁺ and CD11c^–CD138⁺ cells were sorted from cGVHD mice and cultured for 7 days in the presence of the TLR4 (lipopolysaccharide; LPS) or TLR7 agonist (R848). Antichromatin total IgG (a) and antichromatin IgG subclass (b) in the supernatant were subsequently measured by ELISA. In (a), the x-axis shows the dilution factors. Bars represent mean (± SD) of three independent experiments. *P < 0.05, **P < 0.01. IgG immunoglobulin G, ns not significant, OD optical density