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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: T-bet+CD11c+ B cells are critical for antichromatin immunoglobulin G production in the development of lupus

Fig. 4

Antichromatin IgG production during cGVHD required T-bet+CD11c+CD19+ B cells. a CD19+ cells and CD19 cells of the spleen were isolated from B6 mice transferred with splenocytes from Bm12 or B6 mice at 14 days to examine T-bet expression. b Flow cytometric analysis of T-bet+CD11c+CD19+ B cells in the total spleen at day 14 with chronic graft-versus-host disease (cGVHD) induction. c Design of the T-bet-deficient B-cell study. B cells of knockout T-bet were transferred into μMT mice (n = 10). The mice were then intraperitoneally (i.p.) injected with 5 × 107 Bm12 splenocytes. Spleen and serum were collected at 14 days after cGVHD induction. d Flow cytometric analysis of T-bet+CD11c+CD19+ B cells in the total spleen. e ELISA was used to measure the production of sera antichromatin IgG and IgG subclass. Results in (a) are representative of three independent experiments. Values are shown as the mean ± SD. **P < 0.01, ***P < 0.001. IgG immunoglobulin G, ns not significant, BM bone marrow, OD optical density, WT wild-type

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