Fig. 7

Enhanced localization of Toll-like receptor 7 (TLR7) in late endosomal and lysosomal compartments in lupus plasmacytoid dendritic cells (pDCs). (a-h) DCs from healthy controls (HC) or patients with systemic lupus erythematosus (SLE) stained with antibodies to BDCA2, TLR7, KDEL and EEA1, Rab7, LAMP1 and DAPI (blue). Representative images of BDCA2-positive pDCs from HC and pPatients with SLE stained with TLR7 (green) and indicated markers (red) of endocytic pathways are shown (a, c, e, g). Scale bars indicate 5.0 μm and white arrows indicate robust co-localization of TLR7 with Rab7 and LAMP1. Quantificaion of co-localization between TLR7 and KDEL (b), EEA1 (d), Rab7 (f) and LAMP1 (h) is shown. Each symbol represents the value of one individual. The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. The Mann-Whitney test was used to test for the differences between pDCs from HC and patients with SLE