Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease

Perazzio, Sandro Félix; Soeiro-Pereira, Paulo Vitor; dos Santos, Viviane Cardoso; de Brito, Marlon Vilela; Salu, Bruno; Oliva, Maria Luiza Vilela; Stevens, Anne Margherite; de Souza, Alexandre Wagner Silva; Ochs, Hans D.; Torgerson, Troy R.; Condino-Neto, Antonio; Andrade, Luis Eduardo Coelho

doi:10.1186/s13075-017-1443-5

Table 2 Nicotinamide adenine dinucleotide phosphate oxidase subunit protein expression in neutrophils and monocytes

From: Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease

Cell source	Stimulus
Cell source	None	PMA	HC plasma	iBD plasma	aBD plasma
Neutrophils
gp91-phox
HC	23.98 ± 11.55	399.71 ± 47.86^a	148.26 ± 29.29	261.27 ± 32.52^b	271.33 ± 44.23^b
iBD	19.66 ± 11.23	402.00 ± 71.57^a	156.95 ± 31.23	291.87 ± 45.39^b	297.35 ± 22.48^b
aBD	32.68 ± 17.61	464.71 ± 48.45^a	139.05 ± 32.75	277.88 ± 41.76^b	278.64 ± 39.95^b
p22
HC	20.93 ± 11.40	453.04 ± 70.51^a	139.25 ± 31.37	262.56 ± 36.43^b	281.02 ± 53.86^b
iBD	23.72 ± 12.25	438.87 ± 52.98^a	132.62 ± 17.28	279.12 ± 38.67^b	282.62 ± 35.75^b
aBD	38.82 ± 24.88	422.75 ± 64.76^a	152.78 ± 9.32	288.74 ± 30.77^b	314.94 ± 40.60^b
p40
HC	22.73 ± 9.96	377.33 ± 115.38^a	165.77 ± 33.20	279.88 ± 38.13^b	299.00 ± 49.97^b
iBD	42.43 ± 7.36	453.37 ± 77.82^a	133.37 ± 16.08	279.00 ± 50.57^b	293.12 ± 31.47^b
aBD	28.85 ± 12.85	475.42 ± 79.97^a	160.71 ± 20.86	274.71 ± 55.96^b	253.00 ± 36.56^b
p47
HC	22.16 ± 9.23	383.05 ± 69.88^a	132.90 ± 38.84	247.97 ± 92.38^b	280.63 ± 59.20^b
iBD	35.68 ± 22.66	447.75 ± 41.11^a	151.53 ± 29.26	274.12 ± 50.08^b	270.12 ± 56.07^b
aBD	21.04 ± 7.94	400.00 ± 57.01^a	141.27 ± 34.16	282.12 ± 16.01^b	293.00 ± 50.48^b
p67
HC	32.78 ± 13.00	487.36 ± 93.67^a	162.11 ± 32.10	288.44 ± 42.48^b	296.11 ± 55.36^b
iBD	36.61 ± 15.39	396.50 ± 57.82^a	145.12 ± 30.69	289.50 ± 46.72^b	266.12 ± 54.21^b
aBD	26.42 ± 9.91	425.42 ± 50.71^a	160.00 ± 22.37	296.71 ± 42.42^b	274.57 ± 39.45^b
Monocytes
gp91-phox
HC	29.75 ± 16.20	412.02 ± 68.52^a	154.13 ± 19.00	355.05 ± 107.18^b	421.50 ± 78.28^b
iBD	16.43 ± 5.38	415.43 ± 60.54^a	148.00 ± 33.64	318.06 ± 17.94^b	330.40 ± 15.98^b
aBD	22.41 ± 8.09	468.62 ± 30.17^a	149.41 ± 26.47	263.51 ± 50.51^b	282.71 ± 50.99^b
p22
HC	21.37 ± 12.57	486.14 ± 82.35^a	157.44 ± 40.22	267.42 ± 34.51^b	281.82 ± 39.82^b
iBD	23.00 ± 14.07	442.61 ± 44.55^a	157.41 ± 30.28	281.65 ± 35.56^b	283.66 ± 43.68^b
aBD	27.25 ± 21.68	414.80 ± 33.12^a	129.20 ± 15.01	272.47 ± 29.72^b	235.70 ± 53.94^b
p40
HC	28.21 ± 10.94	467.00 ± 67.57^a	147.22 ± 25.00	286.11 ± 37.36^b	273.77 ± 53.97^b
iBD	54.53 ± 6.55	560.12 ± 74.73^a	142.25 ± 30.90	259.25 ± 42.82^b	289.25 ± 35.70^b
aBD	45.62 ± 9.24	468.75 ± 78.34^a	137.00 ± 25.80	290.37 ± 31.87^b	301.25 ± 44.61^b
p47
HC	27.45 ± 11.87	387.52 ± 45.42^a	155.84 ± 17.92	246.52 ± 37.54^b	263.57 ± 60.32^b
iBD	23.56 ± 15.99	416.77 ± 49.75^a	118.50 ± 27.70	278.71 ± 35.03^b	286.70 ± 49.01^b
aBD	29.49 ± 23.42	427.37 ± 63.68^a	160.71 ± 24.88	264.12 ± 21.63^b	262.01 ± 38.54^b
p67
HC	27.09 ± 17.14	453.77 ± 62.69^a	160.44 ± 27.99	272.55 ± 42.45^b	275.22 ± 46.01^b
iBD	29.01 ± 12.42	420.12 ± 71.30^a	140.37 ± 26.73	281.75 ± 38.49^b	303.75 ± 48.17^b
aBD	29.41 ± 12.61	447.00 ± 67.93^a	142.50 ± 34.80	249.75 ± 39.07^b	269.50 ± 33.04^b

Abbreviations: aBD Active Behçet’s disease, HC Healthy control, iBD Inactive Behçet’s disease, PMA Phorbol 12-myristate 13-acetate
Neutrophils and monocytes were obtained from HCs (n = 9), patients with iBD (n = 8), and patients with aBD (n = 8), and protein expression as determined by mean fluorescence intensity was found to be increased after stimulation with BD plasma
Statistical analysis was performed using one-way analysis of variance with the Bonferroni posttest
^a p < 0.05 compared with unstimulated cells and any other stimulus
^b p < 0.05 compared with unstimulated cells and stimulation with plasma from HCs

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