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Table 2 Nicotinamide adenine dinucleotide phosphate oxidase subunit protein expression in neutrophils and monocytes

From: Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease

Cell source Stimulus
None PMA HC plasma iBD plasma aBD plasma
Neutrophils
 gp91-phox
  HC 23.98 ± 11.55 399.71 ± 47.86a 148.26 ± 29.29 261.27 ± 32.52b 271.33 ± 44.23b
  iBD 19.66 ± 11.23 402.00 ± 71.57a 156.95 ± 31.23 291.87 ± 45.39b 297.35 ± 22.48b
  aBD 32.68 ± 17.61 464.71 ± 48.45a 139.05 ± 32.75 277.88 ± 41.76b 278.64 ± 39.95b
 p22
  HC 20.93 ± 11.40 453.04 ± 70.51a 139.25 ± 31.37 262.56 ± 36.43b 281.02 ± 53.86b
  iBD 23.72 ± 12.25 438.87 ± 52.98a 132.62 ± 17.28 279.12 ± 38.67b 282.62 ± 35.75b
  aBD 38.82 ± 24.88 422.75 ± 64.76a 152.78 ± 9.32 288.74 ± 30.77b 314.94 ± 40.60b
 p40
  HC 22.73 ± 9.96 377.33 ± 115.38a 165.77 ± 33.20 279.88 ± 38.13b 299.00 ± 49.97b
  iBD 42.43 ± 7.36 453.37 ± 77.82a 133.37 ± 16.08 279.00 ± 50.57b 293.12 ± 31.47b
  aBD 28.85 ± 12.85 475.42 ± 79.97a 160.71 ± 20.86 274.71 ± 55.96b 253.00 ± 36.56b
 p47
  HC 22.16 ± 9.23 383.05 ± 69.88a 132.90 ± 38.84 247.97 ± 92.38b 280.63 ± 59.20b
  iBD 35.68 ± 22.66 447.75 ± 41.11a 151.53 ± 29.26 274.12 ± 50.08b 270.12 ± 56.07b
  aBD 21.04 ± 7.94 400.00 ± 57.01a 141.27 ± 34.16 282.12 ± 16.01b 293.00 ± 50.48b
 p67
  HC 32.78 ± 13.00 487.36 ± 93.67a 162.11 ± 32.10 288.44 ± 42.48b 296.11 ± 55.36b
  iBD 36.61 ± 15.39 396.50 ± 57.82a 145.12 ± 30.69 289.50 ± 46.72b 266.12 ± 54.21b
  aBD 26.42 ± 9.91 425.42 ± 50.71a 160.00 ± 22.37 296.71 ± 42.42b 274.57 ± 39.45b
Monocytes
 gp91-phox
  HC 29.75 ± 16.20 412.02 ± 68.52a 154.13 ± 19.00 355.05 ± 107.18b 421.50 ± 78.28b
  iBD 16.43 ± 5.38 415.43 ± 60.54a 148.00 ± 33.64 318.06 ± 17.94b 330.40 ± 15.98b
  aBD 22.41 ± 8.09 468.62 ± 30.17a 149.41 ± 26.47 263.51 ± 50.51b 282.71 ± 50.99b
 p22
  HC 21.37 ± 12.57 486.14 ± 82.35a 157.44 ± 40.22 267.42 ± 34.51b 281.82 ± 39.82b
  iBD 23.00 ± 14.07 442.61 ± 44.55a 157.41 ± 30.28 281.65 ± 35.56b 283.66 ± 43.68b
  aBD 27.25 ± 21.68 414.80 ± 33.12a 129.20 ± 15.01 272.47 ± 29.72b 235.70 ± 53.94b
 p40
  HC 28.21 ± 10.94 467.00 ± 67.57a 147.22 ± 25.00 286.11 ± 37.36b 273.77 ± 53.97b
  iBD 54.53 ± 6.55 560.12 ± 74.73a 142.25 ± 30.90 259.25 ± 42.82b 289.25 ± 35.70b
  aBD 45.62 ± 9.24 468.75 ± 78.34a 137.00 ± 25.80 290.37 ± 31.87b 301.25 ± 44.61b
 p47
  HC 27.45 ± 11.87 387.52 ± 45.42a 155.84 ± 17.92 246.52 ± 37.54b 263.57 ± 60.32b
  iBD 23.56 ± 15.99 416.77 ± 49.75a 118.50 ± 27.70 278.71 ± 35.03b 286.70 ± 49.01b
  aBD 29.49 ± 23.42 427.37 ± 63.68a 160.71 ± 24.88 264.12 ± 21.63b 262.01 ± 38.54b
 p67
  HC 27.09 ± 17.14 453.77 ± 62.69a 160.44 ± 27.99 272.55 ± 42.45b 275.22 ± 46.01b
  iBD 29.01 ± 12.42 420.12 ± 71.30a 140.37 ± 26.73 281.75 ± 38.49b 303.75 ± 48.17b
  aBD 29.41 ± 12.61 447.00 ± 67.93a 142.50 ± 34.80 249.75 ± 39.07b 269.50 ± 33.04b
  1. Abbreviations: aBD Active Behçet’s disease, HC Healthy control, iBD Inactive Behçet’s disease, PMA Phorbol 12-myristate 13-acetate
  2. Neutrophils and monocytes were obtained from HCs (n = 9), patients with iBD (n = 8), and patients with aBD (n = 8), and protein expression as determined by mean fluorescence intensity was found to be increased after stimulation with BD plasma
  3. Statistical analysis was performed using one-way analysis of variance with the Bonferroni posttest
  4. a p < 0.05 compared with unstimulated cells and any other stimulus
  5. b p < 0.05 compared with unstimulated cells and stimulation with plasma from HCs