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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis

Fig. 4

Characterization of adipose tissue area and adipocyte size. Representative sections of synovial membranes (SM) stained with a monoclonal perilipin 1A (PLIN) antibody. Magnification of the selected area is also shown. a Control healthy rabbit; b rabbit fed a high-fat diet (HFD); c osteoarthritic (OA) rabbit; d OA rabbit fed with HFD (OA-HFD); scale bar = 100 μm. e Percentage of adipose tissue area in the SM of each group. f Mean of the adipocyte size in the SM of each group. g Representative western blot of PLIN protein levels expressed in the SM. EZ blue staining was used as protein loading control. h Densitometric analysis of PLIN measurement by western blot expressed as fold induction. Data from individual measurements and the mean and 95% CI are shown (n = 9 − 14 SM per group)

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