Fig. 1From: VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexesImmunoreactive VISTA is detected in human synovium tissue sections and lysates of normal and rheumatoid human synovium. a–c Normal human synovium. d–l RA synovium. a, d, g, j Hematoxylin-and-eosin-stained paraffin tissue sections. b, e, h, k Anti-VISTA peroxidase staining developed with diamino-benzidine. e Arrows denote synovial membrane staining; rectangle encloses area enlarged in (g–i). c, f, i, l Nonspecific isotype control antibody staining developed with diamino-benzidine. j–l Neutrophils in vessel in synovium; arrows denote VISTA-stained neutrophils in (k). m Western blot of synovial tissue lysates from rheumatoid arthritis patients (n = 3) and normal synovium (n = 2) stained with anti-human VISTA antibody or anti-histone H3 as sample loading control. VISTA; V domain-containing Ig suppressor of T-cell activationBack to article page