Fig. 4From: VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexesReduced cell surface C5a receptor expression on blood and bone marrow monocytes, on neutrophils, and on cultured BMDMs in V-KO mice. a Representative histogram of cell surface expression of C5aR on BMDMs from wild-type (WT) and V-KO (V-KO) mice (culture day 5). b FACS of cell surface C5aR expression for cd11b+/Ly6G– monocytes (mono) and cd11b+/Ly6G+ neutrophils (PMN) in peripheral blood and bone marrow of WT and V-KO mice. c Western blot analyses of BMDM cells cultured from WT and V-KO mice on days 3–7 of culture (left) and for day 5 BMDMs (right). d Mean fluorescence intensity (MFI) determined by FACS analysis of cell surface expression of C5aR on day 5 cultured BMDMs from WT (red bars) and V-KO (green bars) mice (n = 6). Student t test (two-tailed): **p < 0.025; *p < 0.05Back to article page