Fig. 4

Effects of PPARβ/δ activation on intracellular Reactive oxygen species (ROS) production in human umbilical cord vein endothelial cells (HUVECs). a ROS production in HUVECs incubated in plasma from patients with systemic lupus erythematosus (SLE) with active nephritis (AN) or healthy controls (Ctrol), in the presence and/or in absence of GW0742 and GSK0660. Values are expressed as mean ± SEM (n = 5–6). b ROS production in control siRNA and siRNA-PPAR-β cells incubated in plasma from patients with AN-SLE or Ctrol for 24 h, in the presence or absence of GW0742 (1 μmol/L). All data are mean ± SEM (n = 8). ROS measured by fluorescence in CM-H2DCFDA-loaded cells. ^* P < 0.05 and ^** P < 0.01 vs Ctrol. ^## P < 0.01vs without PPAR agonist. ⁺⁺ P < 0.01 vs GW0742 column. ^δ P < 0.05 vs Ctrol siRNA-control column