Table 1 Oligonucleotides for real-time RT-PCR
mRNA targets | Descriptions | Sense | Antisense |
|---|---|---|---|
PPARβ/δ | Peroxisome proliferator-activated receptor beta | CATTGAGCCCAAGTTCGAGT | GGTTGACCTGCAGATGGAAT |
BiP | Binding immunoglobulin protein | CGGGATCCATGAAGCTCTCCCTGGTG | CCCAAGCTTGGGCAACTCATCTTTTTCTG |
IRE-1α | Inositol-requiring 1 transmembrane kinase/endonuclease-1α | GAATTCCAATGCCGGCCCGGC | AAGCTTGGAGGGCGTCTGGAGTCAC |
PERK | Eukaryotic translation initiation factor-2α kinase 3 | ATTGCATCTGCCTGGTTAC | GACTCCTTCCTTTGCCTFT |
ATF-6 | Activating transcription factor-6 | CAGGGAGAAGGAACTTGTGA | ACTGACCGAGGAGACGAGA |
CHOP | CCAAT/enhancer binding protein homologous protein | ACCAAGGGAGAACCAGGAAACG | TCACCATTCGGTCAATCAGAGC |
NOX-2 | NOX-2 subunit of NADPH oxidase | CCTAAGATAGCGGTTGATGG | GACTTGAGAATGGATGCGAA |
NOX-4 | NOX-4 subunit of NADPH oxidase | AGTCAGCTCTCTCCTTTCAGG | CTTG-CCCCCTTTGAATAAAT |
Actb | Beta actin | CGGTGAAGGTGACAGCAG | TGTGTGGACTTGGGAGAGG |