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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: CX3CL1 promotes MMP-3 production via the CX3CR1, c-Raf, MEK, ERK, and NF-κB signaling pathway in osteoarthritis synovial fibroblasts

Fig. 3

c-Raf is involved in CX3CL1-mediated MMP-3 production in SFs. a OASFs were incubated with CX3CL1 for the indicated time intervals. c-Raf phosphorylation examined using western blot analysis. b OASFs were pretreated for 30 min with CX3CR1 mAb followed by stimulation with CX3CL1 for 15 min. c-Raf protein levels in the cell lysates determined using western blot analysis. c–e OASFs were pretreated for 30 min with c-Raf inhibitor (GW5074, 10 μM), followed by stimulation with CX3CL1 for 24 h. MMP-3 expression examined using qPCR, western blot analysis, and ELISA. f, g OASFs were transfected for 24 h with c-Raf shRNA followed by stimulation with CX3CL1 for 24 h. MMP-3 expression examined using qPCR and ELISA. Results expressed as mean ± SEM (n = 3). *p < 0.05 compared with control; #p < 0.05 compared with CX3CL1-treated group. MMP matrix metalloproteinase, shRNA small hairpin RNA

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Arthritis Research & Therapy

ISSN: 1478-6362

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