Fig. 3

Cellular infiltration into the skin. a Control-treated MRL/lpr mice display prominent infiltration of the skin by macrophages (green) and T cells (red) (yellow arrows), and IgG deposition (gray), which is significantly reduced in BI-BTK-1-treated mice. b Representative images are taken at × 10 and show mice in the treated and control groups. c The intensities of the macrophage and T cell stains (BI-BTK-1, n = 8; control, n = 9), and the IgG stain (BI-BTK-1, n = 6; control, n = 6) were quantitated using ImageJ. Right panel, total circulating IgG concentrations were measured in terminal serum (BI-BTK-1, n = 10; control, n = 11; p < 0.05). d Sections from control (top panel) and BI-BTK-1-treated (bottom panel) mice were further co-stained with Mac-2 (red) and IBA-1 (green) to assess macrophage activation (× 20) (*p < 0.05, **p < 0.01, ***p < 0.001)