Fig. 3

Two-dimensional PAGE of total proteins extracted from FLSs of patients with RA-sSS and Western blot analysis. a Western blot showing immunostaining of FLS proteins with R-anti-3S-P and anti-KLH, respectively. b Colloidal Coomassie Blue staining of two-dimensional gel of FLS proteins. Protein spots indicated by circles were further identified using matrix-assisted laser desorption/ionization time-of-flight MS. Mascot search results showed protein scores of 580, 1113, and 343 for human caldesmon isoform 2 (Cal), vimentin (Vim), and 60 kDa heat shock protein (HSP-60), respectively. FLS Fibroblast-like synoviocyte, KLH Keyhole limpet hemocyanin, RA Rheumatoid arthritis, R-anti-3S-P Rabbit-anti-secondary Sjögren’s syndrome-associated peptide, sSS Secondary Sjögren’s syndrome