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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes

Fig. 4

ER stress induces apoptosis of MZB1+ cells in target organs resulting in decreased serum dsDNA. a Baseline expression of MZB1 and BiP assessed on BWF1 mice at ages of 23 and 34 weeks and there were elevated levels at 34 weeks of age. b MZB1 and BiP expression in spleens from BWF1 mice (25 weeks of age) before or after indicated time points (hours and days) following TM treatment. Beta-actin used as a loading control. Representative blot of three independent experiments with similar results. c Number of TUNEL-positive cells per HPF in kidney and salivary gland of aged BWFI mice (30 weeks of age) serially taken after TM treatment was quantified. d (Upper) TUNEL-positive cells present among inflammatory cells in the kidney. (Lower) Condensed and fragmented nuclei in MZB1+ cells from BWF1 mice kidney following TM treatment. e Anti-dsDNA antibody concentrations in BWF1 mice (n = 7; 30 weeks of age) measured serially by ELISA following TM treatment. B6 mice (n = 2; 30 weeks of age) used as control. TUNEL, TdT-mediated dUTP nick end labeling; HPF, high-power field; hr, hour; *p<0.05

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