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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Soluble uric acid increases PDZK1 and ABCG2 expression in human intestinal cell lines via the TLR4-NLRP3 inflammasome and PI3K/Akt signaling pathway

Fig. 3

PDZK1 regulated expression and function of ABCG2 in human intestinal cells. Cells transfected with ABCG2 or PDZK1 siRNA or scrambled siRNA for 48 h. Cells then incubated with 6 mg/dl soluble urate or 10 mM NaOH for 24 h. a Relative mRNA levels of PDZK1 and ABCG2 determined by RT-qPCR. Data presented as mean ± SEM. **P < 0.01 and ***P < 0.001, compared to control cells; n = 3. b c Representative western blot analysis of PDZK1 and ABCG2. Protein expression normalized to that of GAPDH. Data are presented as the mean ± SEM. *P<0.05 and **P<0.01, compared to control cells; n = 3. d Efflux function of ABCG2 evaluated by detecting the intracellular fluorescence of e-Fluxx-ID® Green Dye with or without the ABCG2 inhibitor, novobiocin. Tinted histograms show fluorescence of untreated cells, and nontinted histograms show fluorescence of inhibitor-treated cells. MAF value is indicative of corresponding protein activity. ABCG2 ATP-binding cassette transporter, subfamily G, member 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase, PDZK1 PDZ domain containing 1, UA uric acid, NC negative control, siR small interfering RNA

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