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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells

Fig. 4

Fli1+/− dermal fibroblasts may modestly and partially contribute to the induction of T helper type 17 cell (Th17)-like regulatory T cells (Tregs) by interleukin (IL)-6 production. a Evaluation of IL-6 expression in dermal fibroblasts isolated from wild-type (WT) and Fli1+/− mice (n = 10 for each group) by qRT-PCR (left panel) and in dermal fibroblasts isolated from bleomycin-treated WT and Fli1+/− mice (n = 3 for each group) by immunoblotting (right panel). b Results of the chromatin immunoprecipitation assay regarding Fli1 occupation on the IL6 promoter with human dermal fibroblasts (n = 4). c Evaluation by flow cytometry of the effect of IL-6 neutralizing antibody on Tregs cocultured with Fli1+/− dermal fibroblasts (n = 6). Representative results of immunoblotting, agarose gel electrophoresis, and flow cytometry are shown for the right panels of a and b and the right upper panel of c, respectively. Gating strategy for identification of CD4+FoxP3+ Tregs is shown in the leftmost panel of c. In each graph, the relative value compared with the control group is expressed as mean ± SEM. AU Arbitrary units, Fli1 Friend leukemia virus integration 1, IgG Immunoglobulin G, mRNA Messenger RNA

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