Anti-CD11b antibody treatment suppresses the osteoclast generation, inflammatory cell infiltration, and autoantibody production in arthritis-prone FcγRIIB-deficient mice

Table 1 Primer pairs used in qRT-PCR and size of PCR products

Gene for	Forward	Reverse	Size
Gene for	(5’ to 3’)	(5’ to 3’)	(bp)
RANK	GCTGGCTACCACTGGAACTC	GTGCAGTTGGTCCAAGGTTT	182
RANKL	TGTACTTTCGAGCGCAGATG	AGGCTTGTTTCATCCTCCTG	160
OPG	TGAGTGTGAGGAAGGGCGTTA	CCATCTGGACATTTTTTGCAAA	130
MCP-1	AGGTCCCTGTCATGCTTCTG	TCTGGACCCATTCCTTCTTG	249
CX3CL1	CGCGTTCTTCCATTTGTGTA	CTGTGTCGTCTCCAGGACAA	169
RANTES	TCGTGCCCACGTCAAGGAGTATTT	TCTTCTCTGGGTTGGCACACACTT	107
TNFα	TATGGCCCAGACCCTCAC	GGTTGTCTTTGAGATCCATGC	110
IL-6	GAGGATACCACTCCCAACAGACC	AAGTGCATCATCGTTGTTCATACA	141
IL-17	TCTCTGATGCTGTTGCTGCT	GACCAGGATCTCTTGCTGGA	346
IL-10	CAGCCGGGAAGACAATAACT	GTTGTCCAGCTGGTCCTTTG	121
BAFF	TTGTCCAGCAGTTTCACAGC	CCGGTGTCAGGAGTTTGACT	160
IL-1β	GCCCATCCTCTGTGACTCAT	AGGCCACAGGTATTTTGTCG	230
BSF-3	CGAGCCTGACTTCAATCCTC	TACGTCGGAGTTCAGCTGTG	185
IFNγ	AAGACAATCAGGCCATCAGC	ATCAGCAGCGACTCCTTTTC	225
β-actin	TGGGTATGGAATCCTGTGG	GTACTTGCGCTCAGGAGGAG	200

RANK receptor activator of NF-κB, RANKL RANK ligand, OPG osteoprotogerin, MCP-1 monocyte chemotactic protein-1, RANTES regulated on activation, normal T cell expressed and secreted, BAFF B-cell-activating factor of the tumor-necrosis-factor family, BSF-3 B cell stimulating factor, IFN-γ interferon-γ, bp base pairs

ISSN: 1478-6362