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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Adiponectin aggravates bone erosion by promoting osteopontin production in synovial tissue of rheumatoid arthritis

Fig. 3

RAW264.7 migration assay by crystal violet staining. a Rheumatoid arthritis synovial fibroblasts (RASFs) were incubated with various concentrations of adiponectin (AD) (0.1, 1 and 10 μg/mL) in 24-well flat-bottom plates for 72 h. RAW264.7 cells were added to Transwell inserts and co-cultured with the aforementioned RASFs for 24 h. The effect of RAW264.7 migration was examined (n = 3). b Culture medium or RASFs were incubated with or without AD (1 μg/mL) in 24-well flat-bottom plates for 72 h. RAW264.7 cells were added to Transwell inserts and co-cultured with the above culture medium or RASFs for 24 h. The effect of RAW264.7 migration was measured (n = 3). c RASFs were incubated with AD (1 μg/mL) and a serial dilution of OPN (30, 300 and 3000 ng/mL) in 24-well flat-bottom plates for 72 h. RAW264.7 cells were added to Transwell inserts and co-cultured with the above RASFs for 24 h. The effect of RAW264.7 migration was investigated (n = 3). Bars show the mean ± SD; *p < 0.05 vs control. The experiment was repeated three times and representative pictures are shown. Ab, antibodies; OD, optical density

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