Fig. 4

Lenti-shOPN injection significantly attenuated synovial inflammation and bone erosion in mice with adiponectin (AD)-treated collagen-induced arthritis (CIA). a Arthritis severity scores and incidence of CIA development in AD-treated CIA mice were recorded daily after 2nd collagen type II (CII) immunization (n = 5). b Representative photographs of AD-treated CIA mice with or without Lenti-shOPN. c Histologic sections of ankle joints were stained with H&E in the indicated groups and values of histopathological scores are shown. Bars show the mean ± SD (*p < 0.05). d Representative three-dimensional renditions of the ankles and paws scanned by microcomputed tomography (microCT). e-h Quantification of bone mineral density (e), ratio between bone surface and bone volume (f), ratio between bone volume and tissue volume (g) and trabecular number (h) was calculated. Values were analyzed using microCT Skyscan software. Bars show the mean ± SD (*p < 0.05)