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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: MicroRNA-302b negatively regulates IL-1β production in response to MSU crystals by targeting IRAK4 and EphA2

Fig. 2

miR-302b repressed MSU-induced inflammatory response in vivo. Four to seven mice injected subcutaneously with 2 ml of sterile air followed by a second injection of 3 ml of sterile air after 3 days. miR-302b agomir (302b-a) and a negative control (NS-a) injected into air pouches on days 2 and 4. Six days after the first injection, 2 mg of MSU crystals in 0.5 ml of PBS or 0.5 ml of PBS alone injected into air pouches. After 9 h, air pouch fluid harvested by injecting 3 ml PBS. a Schematic of in-vivo air pouch model. b Air pouch membranes dissected and expression of miR-302b detected by QPCR. c Supernatants of air pouch fluid analyzed by ELISA for IL-1β. d After centrifuging, precipitated cells from air pouch fluid were stained with PerCP-CD45 Ab, PE-F4/80 Ab, and APC-Gr-1 Ab. Percentage and cell number of migrated leukocytes, microphages, and neutrophils analyzed using flow cytometry. e Sagittal sections of air pouches fixed in 4% paraformaldehyde and stained with hematoxylin and eosin (H&E). Data shown as means ± SEMs (b, c) or means ± SDEVs (d) (***p < 0.001, **p < 0.01, *p < 0.05 by t test). IL-1β interleukin-1β, PBS phosphate buffered saline, MSU monosodium urate, CTRL control, SSC side scatter, FSC forward scatter

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