Fig. 3

miR-302b inhibited NF-κB and caspase-1 signaling. a THP-1 cells transfected with NS-m or 302b-m for 48 h. Six hours after MSU treatment, phosphorylation level of NF-κB and cleaved caspase-1 p20 detected by western blot analysis. b Air pouch membranes dissected from the mice used for Fig. 2 analyzed by immunoblotting for NF-κB and caspase-1 (p20). Densitometric quantification of western blotting gel shown as fold change under the band. Data represent three experiments. MSU monosodium urate, NS-m negative control mimic, 302b-m miR-302b mimic, 302b-a miR-302b agomir, NS-a negative control agomir