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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: MicroRNA-302b negatively regulates IL-1β production in response to MSU crystals by targeting IRAK4 and EphA2

Fig. 5

Overexpression of miR-302b and interference with EphA2 could inhibit cell migration. THP-1 cells transfected with NS-m, 302b-m, si-NC, or si-EphA2 for 48 h. a THP-1 cells subsequently treated with MSU for another 1 h. Rhodamine phalloidin-labeled F-actin (red) and DAPI-labeled nucleus (blue) analyzed by confocal microscopy. b Transwell assay for THP-1 cells plated on upper cell culture inserts. Data represent three experiments, shown as means ± SEMs (***p < 0.001, *p < 0.05 by t test). CTRL control, MSU monosodium urate, NS-m negative control mimic, 302b-m miR-302b mimic, si-NC negative control siRNA, si-EphA2 EPH receptor A2 siRNA

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