Fig. 5
From: Blockade of TGF-β/Smad signaling by the small compound HPH-15 ameliorates experimental skin fibrosis
The histidine-pyridine-histidine ligand derivative HPH-15 reduces the infiltration of inflammatory CD11b+Ly6Chi monocytes in the early stage of fibrosis and profibrotic M2 macrophages during the late stage. Skin samples from normal, vehicle-treated, and HPH-15-treated mice were harvested 7 or 21 days after bleomycin (BLM) injection. a CD45+CD11b+ cells were gated for analysis of the subsets of macrophages. Representative images (left) and bar graphs (right) of Ly6Chi macrophages on day 7 (b) and CD204+ and CD206+ M2 macrophages on day 21 (c) analyzed by flow cytometry. All values represent mean ± SEM; n = 3 per group. * p ≤ 0.05. FSC-A Forward scatter area