Fig. 2

MiR-146a knockout (KO) mice suffer more severe arthritis than wild-type (WT) mice. a A total of 0.5 mg/20 μL monosodium urate (MSU) crystals were injected into the left rear ankle joints of miR-146a KO and WT B6 mice, while the same volume of PBS was injected into the right ankles. The swelling is expressed as the (left-right)/right ratio and a ratio >0.15 indicated inflammation. Data are expressed as mean ± SEM for five mice per group: **p < 0.01, *p < 0.05, t test. b A total of 1 mg/40 μL MSU suspension was injected into the right footpad of mice, and foot thickness was tested at 0, 6, 24, 48, and 72 h after MSU administration: n = 5 for each group, **p < 0.01, *p < 0.05, t test. c A total of 3 mg/0.5 mL MSU was injected into the peritoneal cavity, and after 2.5 h and 5 h the number of peritoneal exudate cells was counted using a hemocytometer; n = 3; groups were compared using the t test. d Peritoneal exudate cells were stained with anti-F4/80 and anti-CD11b; the F4/80⁻ CD11b ⁺ subset was gated as the neutrophil population; n = 3, groups were compared using the t test