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Table 1 The primers used for quantitative PCR

From: Mice with miR-146a deficiency develop severe gouty arthritis via dysregulation of TRAF 6, IRAK 1 and NALP3 inflammasome

 

Forward sequence (5′–3′)

Reverse sequence (5′–3′)

IL-1β

GGGCCTCAAAGGAAAGAATC

CTCTGCTTGTGAGGTGCTGA

TNF-α

ACAAAGGTGCCGCTAACCACATGT

ATGCTGCTGTTTCAGTCGAAGGCA

NALP3

CGTGGTTTCCTCCTTTTGTATT

CGACCTCCTCTCCTCTCTTCTT

ASC

TCACAGAAGTGGACGGAGTG

TGTCTTGGCTGGTGGTCTCT

Caspase-1

CGTGGAGAGAAACAAGGAGTG

AATGAAAAGTGAGCCCCTGAC

TRAF6

ATTTCATTGTCAACTGGGCA

TGAGTGTCCCATCTGCTTGA

IRAK1

GAGACCCTTGCTGGTCAGAG

GCTACACCCACCCACAGAGT

GAPDH

GGTGAAGGTCGGTGTGAACG

TGTAGACCATGTAGTTGAGGTCA

  1. NALP3 NACHT, LRR and PYD domains-containing protein 3, ASC apoptosis-associated speck-like protein containing a CARD, TRAF6 TNF receptor associated factor 6, IRAK1 interleukin-1 receptor-associated kinase, GAPDH glyceraldehyde-3-phosphate dehydrogenase
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Arthritis Research & Therapy

ISSN: 1478-6362

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