Fig. 2

Effect of MTX on expression of core circadian clock genes in synovial fibroblasts. a Cells treated with MTX (10, 100 nM) or control media for 24 h to measure mRNA expression of Per2, Bmal1, Clock, and Cry1. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on the boxplots using black symbols. *p < 0.05 vs control, n = 5. b Cells treated with MTX (10, 100 nM) or control media for 32 h to observe PER2 expression. c Cells treated with MTX (10, 100 nM) or control media for 32 h to measure PER2 expression by ELISA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentile and single values superimposed on the boxplots using black symbols. **p < 0.01 vs control, n = 5. Bmal1 brain and muscle Arnt-like protein-1, Clock circadian locomotor output cycles kaput, Cry cryptochrome, mRNA messenger RNA, MTX methotrexate, Per period