Fig. 3

Effect of MTX on expression of circadian transcriptional factors PAR bZIP and Bik in synovial fibroblasts. a Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of PAR bZIP gene mRNA (Dbp, Tef, and Hlf) and E4bp4. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. *p < 0.05 vs control, n = 5. b Cells treated with MTX (10, 100 nM) or control media for 24 h to measure expression of Bik gene mRNA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. *p < 0.05 vs control, n = 5. c Cells treated with MTX (10, 100 nM) or control media for 48 h to observe CYTOCHROME C expression. d Cells treated with MTX (10, 100 nM) or control media for 48 h to measure CYTOCHROME C expression by ELISA. Values given with 10th, 25th, 50th (median), 75th, and 90th percentiles and single values superimposed on boxplots using black symbols. *p < 0.05 vs control, n = 5. Bik Bcl-2 interacting killer, Dbp D site of the albumin promoter binding protein, E4bp4 E4-binding protein 4, Hlf hepatic leukemia factor, mRNA messenger RNA, MTX methotrexate, Tef thyrotroph embryonic factor