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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Methotrexate inhibits effects of platelet-derived growth factor and interleukin-1β on rheumatoid arthritis fibroblast-like synoviocytes

Fig. 3

LBH expression in fibroblast-like synoviocytes (FLS) in response to platelet-derived growth factor (PDGF) and IL-1β, with or without methotrexate (MTX). a, b Fold change in mRNA levels of LBH compared to unstimulated was assessed by qPCR in serum-starved FLS after stimulation for 12 h with PDGF (10 ng/mL) and/or IL-1β (2 ng/mL) (a), or IL-1β at different concentrations (0.2–20 ng/mL) (b). c Fold change in LBH mRNA levels was measured after 48 h of mitogen stimulation (PDGF 20 ng/mL + IL-1β 2 ng/mL) in the presence or absence of MTX at various doses (0.01–10 μM; n = 2). d, e Treatment with 1 μM MTX upon PDGF 20 ng/mL + IL-1β 2 ng/mL stimulation was evaluated in rheumatoid arthritis (RA) FLS (d) and in osteoarthritis (OA) FLS (e). Values are the mean fold change ± SEM of three to six different FLS lines. *p < 0.05, **p < 0.01, ***p < 0.0001

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