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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Interleukin-37 is increased in adult-onset Still’s disease and associated with disease activity

Fig. 3

Interleukin (IL)-37 inhibits the expression of proinflammatory cytokines in peripheral blood mononuclear cells (PBMCs) of patients with adult-onset Still’s disease (AOSD). PBMCs of patients with AOSD (n = 10) were stimulated with human recombinant IL-37 (100 ng/ml) for 24 h, then incubated further with lipopolysaccharide (LPS) (1 μg/ml) for 4 h. ae The total RNA was harvested for qRT-PCR analysis or incubated further with LPS (1 μg/ml) for 8 h. fj The total protein was harvested for enzyme-linked immunosorbent assay analysis. The cells were further stimulated with 5 mM adenosine triphosphate (ATP) for 20 minutes for IL-18 messenger RNA (e) and protein (j) analysis. The data represent the mean ± SD. * P < 0.05, ** P < 0.01 by Student’s t test. TNF-α Tumor necrosis factor-α

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