Fig. 4

Therapeutic inhibition of Jmjd3 ameliorates both collagenase-induced osteoarthritis (CiOA) pain and disease development. Wild-type (WT) mice were induced with CiOA on day 0 and treated therapeutically from day 20 with PBS, dimethyl sulfoxide (DMSO) or GSK-J4 (0.5 mg/kg i.a.) for 5 consecutive days followed by twice weekly. a Change in weight distribution (pain) over time. b Representative histologic pictures of knee joints (Safranin O/fast green stain, original magnification ×100) and quantification of arthritis at day 42. c Representative histologic pictures of osteophytes (indicated by arrow) (Safranin O/fast green stain; original magnification ×100) and quantification of osteophyte size. Results are expressed as the mean ± SEM; n = 10 mice per strain (from two independent experiments). i.a., intra-articular. *p < 0.05, DMSO vs. GSK-J4