Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

Table 2 Fold change of proteins that are differentially abundant in the synovial fluid of clinical non-responders compared with clinical responders to autologous chondrocyte implantation (ACI) immediately prior to stage I

Protein		Fold change	Identification method
Description	Accession no.	Fold change	LF LC-MS/MS	iTRAQ nLC-MS/MS
Complement C1S subcomponent	P09871	− 5.15		+
Haptoglobin	P00738	− 4.49		+
Mesencephalic astrocyte-derived neurotrophic factor	P55145	2.15		+
Plasma protease C1 inhibitor	P05155	2.19		+
Immunoglobulin kappa chain V-II region MIL	P01615	2.60	+
Bifunctional glutamate/proline-transfer RNA ligase	P07814	2.61		+
Pigment epithelium-derived factor	P36955	3.13		+
Apolipoprotein A-IV	P06727	3.19		+
Apolipoprotein L1	O14791	3.19		+
N-acetylglucosamine-6-sulphatase	P15586	3.25		+
Retinol-binding protein 4	P02753	3.34		+
Inter-alpha-trypsin inhibitor heavy chain H1	P19827	3.37		+
Extracellular matrix protein 1	Q16610	3.77		+
Lumican	P51884	3.80		+
Histidine-rich glycoprotein	P04196	3.84		+
Endoplasmin	P14625	4.37		+
Serum paraoxonase/arylesterase 1	P27169	4.41		+

Footnote: Differential abundance was denoted by greater than or equal to ± 2.0-fold change; p ≤ 0.05; protein identified by at least two unique peptides. Positive numbers denote higher abundance in non-responders than in responders. Proteins were identified using either protein dynamic compression coupled with label-free quantitation LC-MS/MS or no protein dynamic compression with isobaric tags for absolute and relative quantitation (iTRAQ) LC-MS/MS

ISSN: 1478-6362