Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

Table 3 Fold change of proteins that are differentially abundant in the synovial fluid of clinical non-responders compared with clinical responders to autologous chondrocyte implantation (ACI) immediately prior to stage II

Protein		Fold Change	Identification method
Description	Accession	Fold Change	LF LC-MS/MS	iTRAQ nLC-MS/MS
40S Ribosomal protein S14	P62263	− 8.63		+
Kinectin	Q86UP2	− 6.20		+
Apolipoprotein C-III	P02656	− 2.78		+
High-mobility group protein B1	P09429	− 2.56		+
Kininogen-1	P01042	2.27		+
26S Protease regulatory subunit 7	P35998	2.34	+
26S Proteasome non-ATPase regulatory subunit 13	Q9UNM6	2.43	+
Alpha-enolase	P06733	2.56		+
Alpha-2-HS-glycoprotein	P02765	2.78		+
Hemopexin	P02790	2.88		+
Ferritin light chain	P02792	2.91	+
Platelet factor 4	P02776	3.26	+
Thrombospondin-1	P07996	3.40	+
Nucleosome assembly protein 1-like 1	P55209	4.94	+
Cofilin-1	P23528	7.08	+
EH domain-containing protein 1	Q9H4M9	7.30	+
Haemoglobin subunit delta	P02042	8.09		+
Protein S100-A6	P06703	8.39		+
T-complex protein 1 subunit eta	Q99832	8.43	+
Haemoglobin subunit beta	P68871	32.81		+
Haemoglobin subunit alpha	P69905	44.06		+

Footnote: Differential abundance was denoted by greater than or equal to ± 2.0-fold change; p ≤ 0.05; protein identified by at least two unique peptides. Positive numbers denote higher abundance in non-responders than in responders. Proteins were identified using either protein dynamic compression coupled with label-free quantitation LC-MS/MS or no protein dynamic compression with isobaric tags for absolute and relative quantitation (iTRAQ) LC-MS/MS

ISSN: 1478-6362