Fig. 2

JAK2 expression levels are highly upregulated in patients with AS. a JAK2 and IL-17A expression levels were detected by immunohistochemistry (Ct = 3 and AS = 7). Representative image are shown. Scale bar, JAK2 and IL-17A images; 50 μm and 200 μm, respectively. b JAK2 and STAT3 expression in bone tissue were determined by quantitative RT-PCR (Ct = 8 and AS = 8). JAK2 and STAT3 expression levels in BdCs were detected by (c) immunoblotting (Ct-BdCs = 2 and AS-BdCs = 2), (d) quantitative RT-PCR (Ct-BdCs = 8 and AS-BdCs = 8), and (e) immunostaining. Scale bars of confocal image is 50 μm. The Mann-Whitney U test was performed to determine statistical significance. Data are presented as means ± SDs. P values indicate significant differences between the two groups. N.S., not significant; * P < 0.05