Fig. 3
From: IL-17A induces osteoblast differentiation by activating JAK2/STAT3 in ankylosing spondylitis
IL-17A enhances osteogenic activity and differentiation in AS. a ALP activity in serum samples (HC = 23 and AS = 26). b ALP activity in cultured supernatants of Ct-BdCs and AS-BdCs (Ct-BdCs = 6 and AS-BdCs = 6). c ALP and ARS staining of Ct-BdCs and AS-BdCs. d 293 T cells were transfected with ALP-luciferase plasmids and treated with the indicated cytokine for 24 h, after which luciferase activity was analyzed. Representative data are shown (n = 4). Ct-BdCs were seeded and stimulated with various concentrations of IL-17A in the presence of osteogenic medium. Osteogenic changes were then observed by (e) intracellular ALP activity, (f) ARS staining, and (g) immunoblotting for the indicated proteins. The Mann-Whitney U test and one-way ANOVA were performed to determine statistical significance. Data are presented as means ± SDs. P values indicate significant differences between the two groups. N.S., not significant; * P < 0.05; **P < 0.01